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ObjectiveTo investigate the protective effect of salidroside against nonalcoholic fatty liver disease （NAFLD） and its mechanism of action. MethodsA total of 24 male KM mice were randomly divided into normal group， HFD group， HFD+blank control group， and HFD+salidroside group， with 6 mice in each group. The mice in the normal group were given normal diet， and those in the other groups were given high-fat diet. After 14 weeks of modeling， the mice were given salidroside 100 mg/kg/day by gavage， and related samples were collected at the end of week 22. Enzyme-linked immunosorbent assay was used to measure the serum levels of related biochemical parameters including alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， triglyceride （TG）， total cholesterol （TC）， high-density lipoprotein cholesterol （HDL-C）， and low-density lipoprotein cholesterol （LDL-C）； HE staining and NAFLD activity score （NAS） were used to observe the liver histopathology of mice； Western blot was used to measure the changes in the expression of NAMPT， Sirt1， AMPKα， and SREBP1 in liver tissue. A one-way analysis of variance was used for comparison between multiple groups， and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the normal group， the HFD group had obvious steatosis and extensive large lipid droplets in liver tissue， with significant increases in NAS score （P<0.01） and the content of AST， ALT， TG， TC， and LDL-C in peripheral blood （all P<0.05） and a significant reduction in the content of HDL-C （P<0.05）， as well as significant reductions in the expression levels of NAMPT， AMPKα， and Sirt1 in liver tissue （all P<0.05） and a significant increase in the expression level of SERBP1 （P<0.01）. Compared with the HFD group and the HFD+blank control group， the HFD+salidroside group had reductions in the distribution of vacuolar lipid droplets and intralobular inflammation in liver tissue， alleviation of the ballooning degeneration of hepatocytes， significant reductions in NAS score （P<0.01） and the content of AST， ALT， TG， and LDL-C in peripheral blood （all P<0.05）， and a significant increase in the content of HDL-C （P<0.05）， as well as significant increases in the expression levels of NAMPT， AMPKα， and Sirt1 in liver tissue （all P<0.05） and a significant reduction in the expression level of SERBP1 （P<0.01）. ConclusionSalidroside can significantly improve the pathological state of mice with NAFLD induced by high-fat diet and exert a protective effect against NAFLD by increasing the expression of NAMPT， Sirt1， and AMPKα and reducing the expression of SERBP1.
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Objective:To assess the implementation of injection-related risk management based on WeChat platform for type 2 diabetic patients with the insulin self-injection.Methods:A total of 124 diabetic patients, who would receive insulin therapy by self-injection at home after discharge from the PLA 903 hospital during April 2017 to July 2018, were divided into the control group( n=62)and the study group( n=62). All patients were given routine education on insulin injection during the hospitalization,while the study group( n=62)received additional video and text education based on WeChat platform after discharge. The skill of self-injection and the status of blood glucose control were evaluated in both groups 4 weeks and 8 weeks after discharge, respectively. Results:The insulin injection skill, including skin disinfection [36(58%) vs. 11(18%),χ 2=21.42, P<0.01], exhaust before injection [62(100%) vs. 51(82%),χ 2=12.07, P<0.01], stay 10s after injection [60 (97%) vs. 47(76%),χ 2=11.52, P<0.01], disposal of used needle[49(79%) vs. 18(29%),χ 2=31.20, P<0.01], rotation of injection site [48(77%) vs. 35(56%),χ 2=6.16, P=0.01], insulin storage [62(100%) vs. 57(92%),χ 2=5.21, P=0.02], and the ability of correctly dealing with hypoglycemia [52(84%) vs. 38(61%),χ 2=7.94, P=0.01] in the study group were significantly better than those in the control group after 4 weeks of injection-related risk management. The fasting plasma glucose [(6.41±0.76) vs.(7.19±0.81)mmol/L, t=5.61, P<0.01], glycosylated hemoglobin A1c [(6.71±0.64)% vs. (7.37±0.78)%, t=5.18, P<0.01], incidence of hypoglycemia [6(10%) vs. 15(24%),χ 2=4.64, P=0.03] in the study group were significantly lower than those in the control group after 12 weeks of the management. Conclusion:The risk management based on WeChat platform can improve insulin self-injection skill and the ability of dealing with hypoglycemia,also promote effective blood glucose control for diabetes patients.
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Objective:To explore the mental health status of overseas medical students during the COVID-19 epidemic, so as to provide references for the improvement of their mental health level.Methods:Symptom Checklist 90 (SCL-90) was applied to make a survey online among 118 overseas medical students. SPSS 19.0 was used for statistical analysis, and independent sample ttest and univariate analysis were conducted between the groups. Results:The analysis showed that during the COVID-19 epidemic, the scores of SCL-90 factors of overseas medical students in China were significantly higher than those of Chinese youth norms ( P<0.001). The top four scores were as follows: obsessive-compulsive symptoms > paranoia ideation > interpersonal sensitivity > depression. The positive screening rate for SCL-90 of the overseas students was 40.4%. The scores of male overseas students were significantly higher than those of female students ( P<0.01). The scores of overseas students living outside China at present were significantly higher those of students living in China and on campus ( P<0.001). Conclusion:The mental health status of the overseas medical students needs to be attached great importance during the COVID-19 epidemic. Targeted psychological counseling and intervention should be given to foreign students both staying outside China and those on campus.
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Objective To investigate the clinical effect of early holistic treatment in elderly patients with intertrochanteric fracture.Methods From January 2017 to December 2017,84 elderly patients with intertrochanteric fracture received by orthopaedics department of Shaoxing Central Hospital were divided into two groups .The observation group (46 patients) received early holistic therapy ,and the control group (38 patients) received traditional orthopaedic trauma treatment .Two groups of patients were treated with proximal femoral anti -rotation intramedullary nail fixation , and the therapeutic effect was compared between the two groups .Results After12 months of follow -up,the scores of Harris(evaluation standard of total hip joint curative effect ) pain,mobility,deformity,function and total scores in the observation group were (39.13 ±2.66) points,(3.40 ±0.44) points,(3.30 ±0.38) points,(37.73 ± 2.44)points,(83.56 ±2.51) points,respectively,which were higher than those in the control group [(37.05 ± 2.95)points,(3.17 ±0.59)points,(3.09 ±0.52) points,(35.67 ±3.19) points,(78.98 ±3.37) points],there were statistically significant differences between the two groups ( pain:t=3.395,P=0.001;activity:t=2.044,P=0.044;deformity:t=2.135,P=0.035;function:t=3.352,P=0.001;total score:t=7.131,P=0.000).After 12 months of follow-up,the excellent and good rate of operation in the observation group was 84.78％,which was higher than 71.05％in the control group ,there difference was statistically significant between the two groups (χ2 =5.477,P=0.019).The clinical statistics showed that the time from injury to operation ,the time of ambulation and hospitalization in the observation group were (2.31 ±0.53) d,(3.53 ±0.69) d,(15.86 ±2.60) d,respectively, which were shorter than those in the control group [(3.45 ±1.02) d,(5.02 ±1.13) d,(19.73 ±3.12) d],the differences were statistically significant between the two groups (the time from injury to operation:t=6.585,P=0.000;the time of ambulation after operation:7.128,P=0.000;hospitalization time:t =6.202,P=0.000).The incidence of complication was 6.52％in the observation group and 23.68％ in the control group,the difference was statistically significant between the two groups (χ2 =11.484,P=0.000).There was no death in the observation group at 12 months after operation,and the mortality rate in the control group was 2.63％,the difference was no statistically significant between the two groups (χ2 =2.665,P =0.102).Conclusion The application of early holistic treatment concept in the treatment of elderly patients with intertrochanteric fracture can improve the therapeutic effect and shorten the rehabilitation process of the patients .
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Objective@#To investigate the clinical effect of early holistic treatment in elderly patients with intertrochanteric fracture.@*Methods@#From January 2017 to December 2017, 84 elderly patients with intertrochanteric fracture received by orthopaedics department of Shaoxing Central Hospital were divided into two groups.The observation group (46 patients) received early holistic therapy, and the control group (38 patients) received traditional orthopaedic trauma treatment.Two groups of patients were treated with proximal femoral anti-rotation intramedullary nail fixation, and the therapeutic effect was compared between the two groups.@*Results@#After 12 months of follow-up, the scores of Harris(evaluation standard of total hip joint curative effect) pain, mobility, deformity, function and total scores in the observation group were (39.13±2.66)points, (3.40±0.44)points, (3.30±0.38)points, (37.73±2.44)points, (83.56±2.51)points, respectively, which were higher than those in the control group[(37.05±2.95)points, (3.17±0.59)points, (3.09±0.52)points, (35.67±3.19)points, (78.98 ±3.37)points], there were statistically significant differences between the two groups (pain: t=3.395, P=0.001; activity: t=2.044, P=0.044; deformity: t=2.135, P=0.035; function: t=3.352, P=0.001; total score: t=7.131, P=0.000). After 12 months of follow-up, the excellent and good rate of operation in the observation group was 84.78%, which was higher than 71.05% in the control group, there difference was statistically significant between the two groups (χ2=5.477, P=0.019). The clinical statistics showed that the time from injury to operation, the time of ambulation and hospitalization in the observation group were (2.31±0.53) d, (3.53±0.69)d, (15.86±2.60)d, respectively, which were shorter than those in the control group[(3.45±1.02)d, (5.02±1.13)d, (19.73±3.12)d], the differences were statistically significant between the two groups (the time from injury to operation: t=6.585, P=0.000; the time of ambulation after operation: 7.128, P=0.000; hospitalization time: t=6.202, P=0.000). The incidence of complication was 6.52% in the observation group and 23.68% in the control group, the difference was statistically significant between the two groups (χ2=11.484, P=0.000). There was no death in the observation group at 12 months after operation, and the mortality rate in the control group was 2.63%, the difference was no statistically significant between the two groups (χ2=2.665, P=0.102).@*Conclusion@#The application of early holistic treatment concept in the treatment of elderly patients with intertrochanteric fracture can improve the therapeutic effect and shorten the rehabilitation process of the patients.
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AIM To study the chemical constituents from Schisandra chinensis (Turcz.) Baill..METHODS The ethyl acetate fraction of 95％ ethanol extract of S.chinensis was isolated and purified by silica column and recrystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as dodecane (1),palmitic acid (2),arachidic acid (3),β-sitosterol (4),betulinic acid (5),deoxyschisandrin (6),γ-schizandrin (7),schizandrin C (8),gomisin B (9),schisantherin A (10).CONCLUSION Compounds 1 and 5 are isolated from this plant for the first time.